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Image Search Results
Journal: Frontiers in Oncology
Article Title: The Dual Cell Cycle Kinase Inhibitor JNJ-7706621 Reverses Resistance to CD37-Targeted Radioimmunotherapy in Activated B Cell Like Diffuse Large B Cell Lymphoma Cell Lines
doi: 10.3389/fonc.2019.01301
Figure Lengend Snippet: Experimental layout. U-2932 and RIVA cells were treated with 1 or 0.5 μg/mL 177 Lu-lilotomab satetraxetan (600 MBq/mg) for 18 h, excess 177 Lu-lilotomab satetraxetan removed, and cells seeded onto 384-well plates pre-printed with the 384-compound Cambridge Cancer compound library sourced from Selleckchem at final concentrations of 10 nM, 100 nM, or 1 μM. Untreated control cells were seeded on parallel plates. Viability measurements, using RealTime-Glo, were carried out between day 3 and 6 after seeding. Inhibitory compounds were considered as candidate hits if they: (1) in combination with 177 Lu-lilotomab satetraxetan inhibited cell proliferation over two consecutive days to a degree greater than the expected additive effect of the mono-treatments alone (BLISS theorem, see Materials and Methods for details), and (2) drug treatment alone did not reduce viability to <90% of that of untreated control.
Article Snippet: The Selleck Cambridge Cancer Compound library was obtained from and printed onto
Techniques: